Device

Part:BBa_K175045:Design

Designed by: Tim_Weenink   Group: iGEM09_TUDelft   (2009-10-11)


Self Destructive Plasmid with GFP-LVA reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1167
    Illegal NheI site found at 1190
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2776


Design Notes

The restiction site was included only once and outside of any coding region, as the other format was shown to not work.


Source

Assembled in Delft

References